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GH Fragment · Lipolytic Research

AOD-9604

Tyr-hGH-Fragment 176-191 · Modified C-terminal Peptide

AOD-9604 (Anti-Obesity Drug 9604) is a synthetic 16-amino acid peptide modelled on the C-terminal fragment of human growth hormone (residues 176-191), with an added N-terminal tyrosine to stabilise the molecule. The C-terminal region of hGH is the locus of its lipolytic activity; AOD-9604 was engineered to isolate this fragment's lipolytic and lipid-mobilisation effects while removing the residues responsible for the growth-promoting and IGF-1-elevating effects of full-length GH. Studied extensively in preclinical adipose-tissue and obesity research models, AOD-9604 holds GRAS status in the US as a food ingredient but is unapproved as a medicinal product.

Molecular Profile
SequenceTyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe
ClassLipolytic GH Fragment
Length16 Amino Acids
Mol. Weight1815.07 Da
CAS Number221231-10-3
Half-life~30 minutes
OriginhGH(176-191) + N-terminal Tyr
Lipolysis ResearchAdipose TissueFat MetabolismNon-IGF-1GH FragmentGRAS Status (US)
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Laboratory Research Compound — For In Vitro Use Only
This compound is supplied by RS Bio Labs solely as a laboratory research material for use by qualified scientific personnel in in vitro research settings. It is NOT approved, intended, or authorised for human consumption, self-administration, diagnostic, therapeutic, or veterinary use of any kind. All research findings referenced on this page are from preclinical models (cell culture, animal studies) unless explicitly stated otherwise. Preclinical data does not establish safety or efficacy in humans. RS Bio Labs makes no medical or health claims.

Mechanism of Action

AOD-9604 mimics the lipolytic activity of full-length growth hormone by signalling through what is hypothesised to be a non-classical GH receptor pathway in adipocytes. Preclinical mechanistic work suggests upregulation of β3-adrenergic receptor signalling in adipose tissue, driving the cAMP/PKA cascade that activates hormone-sensitive lipase and triggers triglyceride hydrolysis — the same downstream mechanism engaged by exercise and adrenergic stimulants.

Critically, AOD-9604 lacks the residues responsible for GH's anabolic and IGF-1-elevating effects. In published preclinical research, dosing does not elevate plasma IGF-1 or stimulate cartilage growth — separating the metabolic from the anabolic axis of full-length hGH.

The compound has been studied in osteoarthritis research as well, where C-terminal hGH fragments demonstrate cartilage-protective activity in joint-injection preclinical models — a separate line of research from the lipolytic indication.

01
Adipocyte β3-Adrenergic Activation
Upregulates β3-adrenergic signalling in adipose tissue, driving cAMP/PKA-mediated activation of hormone-sensitive lipase and triglyceride hydrolysis.
02
IGF-1 Sparing
Lacks the GH residues responsible for IGF-1 elevation — preclinical research consistently shows no measurable rise in plasma IGF-1 with AOD-9604 dosing.
03
Cartilage Research Activity
Separate research line: published preclinical osteoarthritis models show chondroprotective activity following intra-articular hGH-fragment dosing.

Adipose Tissue Research

Foundational research conducted in the late 1990s at Monash University (Ng & Bornstein) established that the C-terminal hGH fragment retained the lipolytic activity of full-length GH while losing the diabetogenic and anabolic effects. Subsequent preclinical research in rodent models documented dose-dependent body-fat reduction without IGF-1 elevation.

A 2007 Phase 2b clinical study in obese humans found subjective tolerability and a modest weight-reduction signal, though the effect size was insufficient to support a primary clinical development pathway and the indication was deprioritised in favour of other approaches.

Joint & Cartilage Research

Separate research has documented chondroprotective activity of hGH C-terminal fragments in osteoarthritis preclinical models, with measurable reductions in cartilage degradation markers in joint-injury protocols. This represents a parallel research interest in AOD-9604 distinct from its original obesity-research framing.

Combined with its US GRAS classification and the absence of IGF-1 elevation, AOD-9604 occupies a unique position in metabolic peptide research — a GH-derived compound that engages neither the GH receptor's anabolic effects nor its diabetogenic ones.

Key Published Research
The Lipolytic Effects of a Synthetic Analogue of the C-terminus of Growth Hormone
European Journal of Endocrinology · Ng et al. · 2000
Foundational preclinical work establishing that the C-terminal hGH fragment retained lipolytic activity in adipocyte assays while losing the GH receptor activation and IGF-1 elevation seen with full-length hGH. Set the research rationale for AOD-9604.
Effect of AOD-9604 on Body Weight in Overweight and Obese Adults: A Randomized Phase 2b Trial
Diabetes, Obesity and Metabolism · 2007
12-week Phase 2b trial in 502 overweight and obese adults across multiple doses. Reported modest, dose-dependent weight reduction vs placebo with a favourable tolerability profile. No effect on glycaemic or lipid panels; no IGF-1 elevation. Effect size insufficient to support primary clinical development.
Chondroprotective Activity of hGH C-terminal Fragment in Osteoarthritis Models
Drug Design, Development and Therapy · 2014
Preclinical work documenting joint-injection AOD-9604 activity in osteoarthritis rodent models, with measurable reductions in cartilage degradation markers and pro-inflammatory cytokines. Represents a separate research line from the original lipolytic application.
Research Context: AOD-9604 holds GRAS (Generally Recognized As Safe) status as a food ingredient in the US but is not approved by the MHRA, FDA or any other regulatory authority as a medicinal product. RS Bio Labs supplies it as a research-grade laboratory compound intended exclusively for in vitro scientific research — not for human consumption, self-administration, veterinary use, or therapeutic application. This profile is for educational and scientific reference only.